Support Center

Prepare

Last Updated: Jan 07, 2019 07:48AM PST
Prepare runs the Relax protocol, which aggressively minimizes backbone and side chain, while placing very tight constraints on every heavy (non-hydrogen) atom to keep the structure very close to the original crystal structure. So this is more restrained than a normal Relax run. Prepare will find a more favorable conformation in terms of Rosetta Energy. Often, this is a more natural conformation than many input structures because structures from the PDB are prone to unnatural variations due to crystal packing or experimental factors that cause low resolution in portions of the protein.

For Rosetta command-line users, this protocol uses 
     -relax:constrain_relax_to_start_coords
     -relax:coord_constrain_sidechains
 
ef828e56a55e7b26e25ee456cc7df695@cyrusbiotechnology.desk-mail.com
https://cdn.desk.com/
false
desk
Loading
seconds ago
a minute ago
minutes ago
an hour ago
hours ago
a day ago
days ago
about
false
Invalid characters found
/customer/en/portal/articles/autocomplete