by Elizabeth Swanberg | Aug 29, 2019 | Advanced use of Design, Workflows
One of the most effective ways to improve protein stability and solubility is to use evolutionary information to guide mutation selection. This workflow shows how you can create a multi-sequence alignment from homologs in the NCBI database, then sample the mutations...
by Elizabeth Swanberg | Aug 29, 2019 | Advanced use of Design, Workflows
Researchers often have a new sequence they want to model or use as a starting point for future design work. We have a quick and simple method for doing this 1.) Load the protein into CAD Use the as usual. Include or exclude any additional molecules in the file that...
by Elizabeth Swanberg | Jan 20, 2019 | Advanced use of Design, Workflows
After running DDG, users often want to select mutations that are found to be favorable as a mutation set for Design. We are working on adding an automatic implementation for this in CAD, but in the meantime you can download a program that will do it for you. This tool...
by Elizabeth Swanberg | Jan 20, 2019 | Advanced use of Design, Workflows
Workflows for rescoring protein-protein complex from other software, and disulfide bond removal with maintained overall...
by Elizabeth Swanberg | Jan 20, 2019 | Advanced use of Design, Workflows
Download "Using HM & CAD Together"...
